abstract：:Plants are versatile chemists producing a tremendous variety of specialized compounds. Here, we describe the engineering of entirely novel metabolic pathways in planta enabling generation of halogenated indigo precursors as non-natural plant products. Indican (indolyl-β-D-glucopyranoside) is a secondary metabolite cha...

journal_title：Metabolic engineering

authors： Fräbel S,Wagner B,Krischke M,Schmidts V,Thiele CM,Staniek A,Warzecha H

更新日期：2018-03-01 00:00:00

abstract：:Valine, which is one of the branched-chain amino acids (BCAAs) essential for humans, is widely used in animal feed, dietary supplements and pharmaceuticals. At the commercial level, valine is usually produced by bacterial fermentation from glucose. However, valine biosynthesis can also proceed in the yeast Saccharomyc...

journal_title：Metabolic engineering

authors： Takpho N,Watanabe D,Takagi H

更新日期：2018-03-01 00:00:00

abstract：:Building biosynthetic pathways and engineering metabolic reactions in cells can be time-consuming due to complexities in cellular metabolism. These complexities often convolute the combinatorial testing of biosynthetic pathway designs needed to define an optimal biosynthetic system. To simplify the optimization of bio...

journal_title：Metabolic engineering

authors： Karim AS,Heggestad JT,Crowe SA,Jewett MC

更新日期：2018-01-01 00:00:00

abstract：:An engineered reversal of the β-oxidation cycle (r-BOX) and the fatty acid biosynthesis (FAB) pathway are promising biological platforms for advanced fuel and chemical production in part due to their iterative nature supporting the synthesis of various chain length products. While diverging in their carbon-carbon elon...

journal_title：Metabolic engineering

authors： Clomburg JM,Contreras SC,Chou A,Siegel JB,Gonzalez R

更新日期：2018-01-01 00:00:00

abstract：:Caffeic acid has been widely recognized as a versatile pharmacophore for synthesis of new chemical entities, among which caffeic acid derived phenethyl esters and amides are the most extensively-investigated bioactive compounds with potential therapeutical applications. However, the natural biosynthetic routes for caf...

journal_title：Metabolic engineering

authors： Wang J,Mahajani M,Jackson SL,Yang Y,Chen M,Ferreira EM,Lin Y,Yan Y

更新日期：2017-11-01 00:00:00

abstract：:Malonyl-CoA is the basic building block for synthesizing a range of important compounds including fatty acids, phenylpropanoids, flavonoids and non-ribosomal polyketides. Centering around malonyl-CoA, we summarized here the various metabolic engineering strategies employed recently to regulate and control malonyl-CoA ...

journal_title：Metabolic engineering

authors： Johnson AO,Gonzalez-Villanueva M,Wong L,Steinbüchel A,Tee KL,Xu P,Wong TS

更新日期：2017-11-01 00:00:00

abstract：:Polysialic acid (polySia) is a posttranslational modification found on only a handful of proteins in the central nervous and immune systems. The addition of polySia to therapeutic proteins improves pharmacokinetics and reduces immunogenicity. To date, polysialylation of therapeutic proteins has only been achieved in v...

journal_title：Metabolic engineering

authors： Keys TG,Wetter M,Hang I,Rutschmann C,Russo S,Mally M,Steffen M,Zuppiger M,Müller F,Schneider J,Faridmoayer A,Lin CW,Aebi M

更新日期：2017-11-01 00:00:00

abstract：:Succinic acid is a platform chemical of recognized industrial value and accordingly faces a continuous challenge to enable manufacturing from most attractive raw materials. It is mainly produced from glucose, using microbial fermentation. Here, we explore and optimize succinate production from sucrose, a globally appl...

journal_title：Metabolic engineering

authors： Lange A,Becker J,Schulze D,Cahoreau E,Portais JC,Haefner S,Schröder H,Krawczyk J,Zelder O,Wittmann C

更新日期：2017-11-01 00:00:00

abstract：:Pigmented metabolites have great potential for use in biosensors that target low-resource areas, since sensor output can be interpreted without any equipment. However, full repression of pigment production when undesired is challenging, as even small amounts of enzyme can catalyze the production of large, visible amou...

journal_title：Metabolic engineering

authors： McNerney MP,Styczynski MP

更新日期：2017-09-01 00:00:00

abstract：:Isobutanol is a valuable chemical and is considered a new generation biofuel. Construction of isobutanol synthesis pathways in bacteria is a hot topic in isobutanol production. Here, we show that an isobutanol synthesis pathway exists naturally in Klebsiella pneumoniae; however, this pathway is dormant in the wild-typ...

journal_title：Metabolic engineering

authors： Gu J,Zhou J,Zhang Z,Kim CH,Jiang B,Shi J,Hao J

更新日期：2017-09-01 00:00:00

abstract：:Mutations in succinate dehydrogenase (SDH) are associated with tumor development and neurodegenerative diseases. Only in tumors, loss of SDH activity is accompanied with the loss of complex I activity. Yet, it remains unknown whether the metabolic phenotype of SDH mutant tumors is driven by loss of complex I function,...

journal_title：Metabolic engineering

authors： Lorendeau D,Rinaldi G,Boon R,Spincemaille P,Metzger K,Jäger C,Christen S,Dong X,Kuenen S,Voordeckers K,Verstreken P,Cassiman D,Vermeersch P,Verfaillie C,Hiller K,Fendt SM

更新日期：2017-09-01 00:00:00

abstract：:N-linked glycosylation of proteins has both functional and structural significance. Importantly, the glycan structure of a therapeutic protein influences its efficacy, pharmacokinetics, pharmacodynamics and immunogenicity. In this work, we developed glycosylation flux analysis (GFA) for predicting intracellular produc...

journal_title：Metabolic engineering

authors： Hutter S,Villiger TK,Brühlmann D,Stettler M,Broly H,Soos M,Gunawan R

更新日期：2017-09-01 00:00:00

abstract：:β-Oxidation is the ubiquitous metabolic strategy to break down fatty acids. In the course of this four-step process, two carbon atoms are liberated per cycle from the fatty acid chain in the form of acetyl-CoA. However, typical β-oxidative strategies are not restricted to monocarboxylic (fatty) acid degradation only, ...

journal_title：Metabolic engineering

authors： Kallscheuer N,Polen T,Bott M,Marienhagen J

更新日期：2017-07-01 00:00:00

abstract：:Ginsenoside Rh2 is a potential anticancer drug isolated from medicinal plant ginseng. Fermentative production of ginsenoside Rh2 in yeast has recently been investigated as an alternative strategy compared to extraction from plants. However, the titer was quite low due to low catalytic capability of the key ginseng gly...

journal_title：Metabolic engineering

authors： Zhuang Y,Yang GY,Chen X,Liu Q,Zhang X,Deng Z,Feng Y

更新日期：2017-07-01 00:00:00

abstract：:Artificial control of bio-functions through regulating gene expression is one of the most important and attractive technologies to build novel living systems that are useful in the areas of chemical synthesis, nanotechnology, pharmacology, cell biology. Here, we present a novel real-time control system of gene regulat...

journal_title：Metabolic engineering

authors： Wang J,Cui X,Yang L,Zhang Z,Lv L,Wang H,Zhao Z,Guan N,Dong L,Chen R

更新日期：2017-07-01 00:00:00

abstract：:High titer 1-butanol production in Escherichia coli has previously been achieved by overexpression of a modified clostridial 1-butanol production pathway and subsequent deletion of native fermentation pathways. This strategy couples growth with production as 1-butanol pathway offers the only available terminal electro...

journal_title：Metabolic engineering

authors： Ohtake T,Pontrelli S,Laviña WA,Liao JC,Putri SP,Fukusaki E

更新日期：2017-05-01 00:00:00

abstract：:Ethyl acetate is an industrially relevant ester that is currently produced exclusively through unsustainable processes. Many yeasts are able to produce ethyl acetate, but the main responsible enzyme has remained elusive, hampering the engineering of novel production strains. Here we describe the discovery of a new enz...

journal_title：Metabolic engineering

authors： Kruis AJ,Levisson M,Mars AE,van der Ploeg M,Garcés Daza F,Ellena V,Kengen SWM,van der Oost J,Weusthuis RA

更新日期：2017-05-01 00:00:00

abstract：:Biotin is an archetypal vitamin used as cofactor for carboxylation reactions found in all forms of life. However, biotin biosynthesis is an elaborate multi-enzymatic process and metabolically costly. Moreover, many industrially relevant organisms are incapable of biotin synthesis resulting in the requirement to supple...

journal_title：Metabolic engineering

authors： Jeschek M,Bahls MO,Schneider V,Marlière P,Ward TR,Panke S

更新日期：2017-03-01 00:00:00

abstract：:Actinomycetes produce a large variety of pharmaceutically active compounds, yet production titers often require to be improved for discovery, development and large-scale manufacturing. Here, we describe a new technique, multiplexed site-specific genome engineering (MSGE) via the 'one integrase-multiple attB sites' con...

journal_title：Metabolic engineering

authors： Li L,Zheng G,Chen J,Ge M,Jiang W,Lu Y

更新日期：2017-03-01 00:00:00

abstract：:The introduction of a synthetic metabolic pathway consisting of multiple genes derived from various organisms enables cyanobacteria to directly produce valuable chemicals from carbon dioxide. We previously constructed a synthetic metabolic pathway composed of genes from Escherichia coli, Saccharomyces cerevisiae, and ...

journal_title：Metabolic engineering

authors： Hirokawa Y,Maki Y,Hanai T

更新日期：2017-01-01 00:00:00

abstract：:Current studies on microbial isoprene biosynthesis have mostly focused on regulation of the upstream mevalonic acid (MVA) or methyl-erythritol-4-phosphate (MEP) pathway. However, the downstream bottleneck restricting isoprene biosynthesis capacity caused by the weak expression and low activity of plant isoprene syntha...

journal_title：Metabolic engineering

authors： Wang F,Lv X,Xie W,Zhou P,Zhu Y,Yao Z,Yang C,Yang X,Ye L,Yu H

更新日期：2017-01-01 00:00:00

abstract：:Synthesis and accumulation of plant oils in the entire vegetative biomass offers the potential to deliver yields surpassing those of oilseed crops. However, current levels still fall well short of those typically found in oilseeds. Here we show how transcriptome and biochemical analyses pointed to a futile cycle in a ...

journal_title：Metabolic engineering

authors： Vanhercke T,Divi UK,El Tahchy A,Liu Q,Mitchell M,Taylor MC,Eastmond PJ,Bryant F,Mechanicos A,Blundell C,Zhi Y,Belide S,Shrestha P,Zhou XR,Ral JP,White RG,Green A,Singh SP,Petrie JR

更新日期：2017-01-01 00:00:00

abstract：:Microbial fermentation conditions are dynamic, due to transcriptional induction, nutrient consumption, or changes to incubation conditions. In this study, 13C-metabolic flux analysis was used to characterize two violacein-producing E. coli strains with vastly different productivities, and to profile their metabolic ad...

journal_title：Metabolic engineering

authors： He L,Xiu Y,Jones JA,Baidoo EEK,Keasling JD,Tang YJ,Koffas MAG

更新日期：2017-01-01 00:00:00

abstract：:Cyanobacteria hold promise as a cell factory for producing biofuels and bio-derived chemicals, but genome engineering of cyanobacteria such as Synechococcus elongatus PCC 7942 poses challenges because of their oligoploidy nature and long-term instability of the introduced gene. CRISPR-Cas9 is a newly developed RNA-gui...

journal_title：Metabolic engineering

authors： Li H,Shen CR,Huang CH,Sung LY,Wu MY,Hu YC

更新日期：2016-11-01 00:00:00

abstract：:Branched-chain fatty acids (BCFAs) are key precursors of branched-chain fuels, which have cold-flow properties superior to straight chain fuels. BCFA production in Gram-negative bacterial hosts is inherently challenging because it competes directly with essential and efficient straight-chain fatty acid (SCFA) biosynth...

journal_title：Metabolic engineering

authors： Bentley GJ,Jiang W,Guamán LP,Xiao Y,Zhang F

更新日期：2016-11-01 00:00:00

abstract：:Isoprene is facing a growing global market due to its wide industrial applications. Current industrial production of isoprene is almost entirely petroleum-based, which is influenced by the shrinking C5 supply, while the natural emission of isoprene is predominantly contributed by plants. To bridge the need gap, a high...

journal_title：Metabolic engineering

authors： Ye L,Lv X,Yu H

更新日期：2016-11-01 00:00:00

abstract：:13C metabolic flux analysis (13C-MFA) is a widely used tool for quantitative analysis of microbial and mammalian metabolism. Until now, 13C-MFA was based mainly on measurements of isotopic labeling of amino acids derived from hydrolyzed biomass proteins and isotopic labeling of extracted intracellular metabolites. Her...

journal_title：Metabolic engineering

authors： Long CP,Au J,Gonzalez JE,Antoniewicz MR

更新日期：2016-11-01 00:00:00

abstract：:We present a model-based method, designated Inverse Metabolic Control Analysis (IMCA), which can be used in conjunction with classical Metabolic Control Analysis for the analysis and design of cellular metabolism. We demonstrate the capabilities of the method by first developing a comprehensively curated kinetic model...

journal_title：Metabolic engineering

authors： Savoglidis G,da Silveira Dos Santos AX,Riezman I,Angelino P,Riezman H,Hatzimanikatis V

更新日期：2016-09-01 00:00:00

abstract：:Synthesis of polyketides at high titer and yield is important for producing pharmaceuticals and biorenewable chemical precursors. In this work, we engineered cofactor and transport pathways in Saccharomyces cerevisiae to increase acetyl-CoA, an important polyketide building block. The highly regulated yeast pyruvate d...

journal_title：Metabolic engineering

authors： Cardenas J,Da Silva NA

更新日期：2016-07-01 00:00:00

abstract：:Rational metabolic engineering methods are increasingly employed in designing the commercially viable processes for the production of chemicals relevant to pharmaceutical, biotechnology, and food and beverage industries. With the growing availability of omics data and of methodologies capable to integrate the availabl...

journal_title：Metabolic engineering

authors： Andreozzi S,Chakrabarti A,Soh KC,Burgard A,Yang TH,Van Dien S,Miskovic L,Hatzimanikatis V

更新日期：2016-05-01 00:00:00

abstract：:Acid-tolerant Saccharomyces cerevisiae was engineered to produce lactic acid by expressing heterologous lactate dehydrogenase (LDH) genes, while attenuating several key pathway genes, including glycerol-3-phosphate dehydrogenase1 (GPD1) and cytochrome-c oxidoreductase2 (CYB2). In order to increase the yield of lactic ...

journal_title：Metabolic engineering

authors： Song JY,Park JS,Kang CD,Cho HY,Yang D,Lee S,Cho KM

更新日期：2016-05-01 00:00:00

abstract：:An efficient production process is important for industrial microorganisms. The cellular efficiency of solventogenic clostridia, a group of anaerobes capable of producing a wealth of bulk chemicals and biofuels, must be improved for competitive commercialization. Here, using Clostridium acetobutylicum, a species of so...

journal_title：Metabolic engineering

authors： Yang Y,Lang N,Yang G,Yang S,Jiang W,Gu Y

更新日期：2016-05-01 00:00:00

abstract：:One of the key drivers for successful metabolic engineering in microbes is the efficacy by which genomes can be edited. As such there are many methods to choose from when aiming to modify genomes, especially those of model organisms like yeast and bacteria. In recent years, clustered regularly interspaced palindromic ...

journal_title：Metabolic engineering

authors： Jakočiūnas T,Jensen MK,Keasling JD

更新日期：2016-03-01 00:00:00

abstract：:Development of sustainable biological process for the production of bulk chemicals from renewable feedstock is an important goal of white biotechnology. Ethylene glycol (EG) is a large-volume commodity chemical with an annual production of over 20 million tons, and it is currently produced exclusively by petrochemical...

journal_title：Metabolic engineering

authors： Chen Z,Huang J,Wu Y,Liu D

更新日期：2016-01-01 00:00:00

abstract：:We have isolated a new extremely thermophilic fast-growing Geobacillus strain that can efficiently utilize xylose, glucose, mannose and galactose for cell growth. When grown aerobically at 72 °C, Geobacillus LC300 has a growth rate of 2.15 h(-1) on glucose and 1.52 h(-1) on xylose (doubling time less than 30 min). The...

journal_title：Metabolic engineering

authors： Cordova LT,Long CP,Venkataramanan KP,Antoniewicz MR

更新日期：2015-11-01 00:00:00

abstract：:Metabolic models used in 13C metabolic flux analysis generally include a limited number of reactions primarily from central metabolism. They typically omit degradation pathways, complete cofactor balances, and atom transition contributions for reactions outside central metabolism. This study addresses the impact on pr...

journal_title：Metabolic engineering

authors： Gopalakrishnan S,Maranas CD

更新日期：2015-11-01 00:00:00

abstract：:Predicting resource allocation between cell processes is the primary step towards decoding the evolutionary constraints governing bacterial growth under various conditions. Quantitative prediction at genome-scale remains a computational challenge as current methods are limited by the tractability of the problem or by ...

journal_title：Metabolic engineering

authors： Goelzer A,Muntel J,Chubukov V,Jules M,Prestel E,Nölker R,Mariadassou M,Aymerich S,Hecker M,Noirot P,Becher D,Fromion V

更新日期：2015-11-01 00:00:00

abstract：:Cyanobacteria can be exploited as photosynthetic platforms for heterologous generation of terpene hydrocarbons with industrial applications. Transformation of Synechocystis and heterologous expression of the β-phellandrene synthase (PHLS) gene alone is necessary and sufficient to confer to Synechocystis the ability to...

journal_title：Metabolic engineering

authors： Formighieri C,Melis A

更新日期：2015-11-01 00:00:00

abstract：:Engineering cellular metabolism for improved production of valuable chemicals requires extensive modulation of bacterial genome to explore complex genetic spaces. Here, we report the development of a CRISPR-Cas9 based method for iterative genome editing and metabolic engineering of Escherichia coli. This system enable...

journal_title：Metabolic engineering

authors： Li Y,Lin Z,Huang C,Zhang Y,Wang Z,Tang YJ,Chen T,Zhao X

更新日期：2015-09-01 00:00:00

abstract：:Aromatic amino acids are precursors of numerous plant secondary metabolites with diverse biological functions. Many of these secondary metabolites are already being used as active pharmaceutical or nutraceutical ingredients, and there are numerous exploratory studies of other compounds with promising applications. p-C...

journal_title：Metabolic engineering

authors： Rodriguez A,Kildegaard KR,Li M,Borodina I,Nielsen J

更新日期：2015-09-01 00:00:00